In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope.
This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens.
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In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens.
Without oil, light waves reflect off the slide specimen through the glass cover slip, through the air, and into the microscope lens